High-affinity TCRs

High-Affinity Public TCRs within Human Selection of T Cell Clones Expressing

Transnuclear TRP1-specific CD8 T cells with high or low affinity TCRs show equivalent antitumor activity.

We have generated, via somatic cell nuclear transfer, two independent lines of transnuclear (TN) mice, using as nuclear donors CD8 T cells, sorted by tetramer staining, that recognize the endogenous melanoma antigen TRP1. These two lines of nominally identical specificity differ greatly in their affinity for antigen (TRP1(high) or TRP1(low)) as inferred from tetramer dissociation and peptide re...

Antibody Stabilization of Peptide–MHC Multimers Reveals Functional T Cells Bearing Extremely Low-Affinity TCRs

Fluorochrome-conjugated peptide-MHC (pMHC) multimers are commonly used in combination with flow cytometry for direct ex vivo visualization and characterization of Ag-specific T cells, but these reagents can fail to stain cells when TCR affinity and/or TCR cell-surface density are low. pMHC multimer staining of tumor-specific, autoimmune, or MHC class II-restricted T cells can be particularly ch...

TCRs alive on the periphery

TCRs alive on the periphery eeping T cell receptors (TCRs) away from the center of the immunological synapse boosts stimulatory stignals, based on work from The immunological synapse—the cell–cell junction between a T cell and antigen-presenting cell (APC)—looks like a bull's eye, with a central cluster of TCRs and their bound antigen–MHC ligands surrounded by a ring of adhesion molecules and t...

S78. Proffered paper: High-affinity CD20-specific TCRs suitable for adoptive immunotherapy can be readily isolated from the allo-repertoire using reverse immunology

Studies using T-cell receptor (TCR) or chimeric antigen receptor (CAR) transduced T-cells have shown the effectiveness of adoptive immunotherapy to treat different malignancies. The efficacy and safety of such interventions greatly depends on good target selection to prevent ontarget toxicity. Furthermore, the broad application of TCR-based adoptive immunotherapy is hampered by a lack of an eff...